FAST in Corynebacterium glutamicum adds to the long list of microorganisms already FAST-equipped for a variety of purposes: metabolic engineering, screening, virulence mechanism deciphering, etc.
Indeed, the team led by Prof. Ki Jun Jeong of KAIST has just disclosed in Microb. Cell Fact. 2024 FAST for screening Corynebacterium glutamicum towards the secretory production of recombinant proteins, including high-value industrial enzymes and therapeutic proteins.
First, the authors constructed a signal peptide library. Then, screening those in C. glutamicum, they achieved improved production of target proteins compared to well-known signal peptides. As a result they believe C. glutamicum to be a most promising workhorse for the bioindustry beyond single aminoacids.
FAST in Corynebacterium glutamicum – (c) 2024 The Authors (from Microb. Cell Fact.)
Of the several assays used for screening, one implements FAST, the fluorescent reporting system now well-known in microbiology. The authors found FAST fusions most relevant for non activity-based screening of non-enzymatic proteins of interest. They used TFAMber-NP, a membrane non-permeant fluorogen, as a cognate ligand, hence selectively recognizing excreted proteins and no cytoplasmic targets.
Read more about FAST in Corynebacterium glutamicum
Jeon, E.J., Lee, S.M., Hong, H.S. et al. Design of fully synthetic signal peptide library and its use for enhanced secretory production of recombinant proteins in Corynebacterium glutamicum. Microb Cell Fact 23, 252 (2024). doi.org/10.1186/s12934-024-02516-9
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